An improved method of analysing 2,5-hexanedione in urine.
نویسندگان
چکیده
A short gas-chromatographic method for analysing urinary concentrations of 2,5-hexanedione is based on acid hydrolysis of urine at pH below 0.1 and "purification" of the urine samples by microcolumns containing an octadecyl-silane phase. A 5% acetonitrile solution allows a fairly selective elution of 2,5-hexanedione from the microcolumns. Recovery of 2,5-hexanedione from urine is as great as 79.9%. The variation coefficient of the measurements is 2.8%. The results obtained from different working conditions and using packed or wide bore or capillary gas-chromatographic columns are reported.
منابع مشابه
Determination of urinary 2,5-hexanedione concentration by an improved analytical method as an index of exposure to n-hexane.
2,5-Hexanedione is a main metabolite of n-hexane and is considered as the cause of n-hexane polyneuropathy. Therefore, it is useful to measure 2,5-hexanedione for biological monitoring of exposure to n-hexane. The analytical methods existing for n-hexane metabolites, however, were controversial and not established enough. Hence, a simple and precise method for determination of urinary 2,5-hexan...
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In order to determine the optimal conditions of acid hydrolysis for urinary 2,5-hexanedione (HD) measurement, the effects of urine pH or volume of HCl added to urine and hydrolysis period were studied using gas chromatography-mass spectrometry (GC-MS). When 0.3 ml of concentrated HCl was added to 3 ml of urine, complete hydrolysis to liberate HD was attained 2 h after the start of heating at 10...
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The physiologicomathematical model with eight compartments described allows the simulation of the absorbtion, distribution, biotransformation, excretion of an organic solvent, and the kinetics of its metabolites. The usual compartments of the human organism (vessel rich group, muscle group, and fat group) are integrated with the lungs, the metabolising tissues, and three other compartments deal...
متن کاملUrinary 2,5-hexanedione excretion in cryptogenic polyneuropathy compared to the general Swedish population
BACKGROUND 2,5-hexanedione (2,5-HD) is the main neurotoxic metabolite of methyl-n-butyl ketone (MBK) and n-hexane, and known to cause polyneuropathy. The aim of our study was to compare the urinary levels of 2,5-HD between cases with cryptogenic polyneuropathy and the general Swedish population, and to elucidate the role of certain external factors. METHODS Morning urine samples were collecte...
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ورودعنوان ژورنال:
- British journal of industrial medicine
دوره 47 6 شماره
صفحات -
تاریخ انتشار 1990